2. Scientists and Bioinformaticians. • Interested in Experimental Design and De Novo assembly using. the PacBio® HGAP method. After the training, you will be able to. • Understand how the HGAP method works. • Understand the coverage targets for de novo assembly with PacBio. data. • Import and parameterize an HGAP assembly job in SMRT ...This video provides an overview of the basic types of DNA size selection protocols on the Pippin systems, and how they are programmed in the software interface.The entire elution was size selected on a 3% agarose cassette using the Blue Pippin system, targeting either 190 or 200 bp using “tight” settings. The 40 μL elution was collected from blue pippin and analyzed using High Sensitivity DNA bioanalyzer. Dilution is usually not necessary for this pool, but think about your expected concentration ...User Guide BUF-7510 Sage Science Part Nos: Sage Science, Inc. Suite 2400 500 Cummings Center Beverly, MA 01915 TM High-PassTM DNA Size Selection BLF-7510 doc# 460046Blue clouds of poisonous gas. Relentless shelling and machine-gun fire. Horace Pippin's journals recorded life in 'them lonely, cooty, muddy trenches.'from BioRad and Pippin Pulse System from Sage Science). Figure 2. Low DNA Input Sample and SMRTbell Library Preparation Workflow. To sequence a single low DNA input sample, ≥400 ng of input gDNA is required for the Sequel II System and the target DNA shear size distribution is 12 kb - 20 kb using the Megaruptor system. The library preparationFind many great new & used options and get the best deals for Sage Science BluePippin BR02552 50bp-30kb DNA Size Selection System Blue Pippin at the best online prices at eBay! Free shipping for many products!The Blue Pippin was set to select a size range of 15-50 kb. After collection of the desired fraction, a 1× AMPure XP bead cleanup was performed. The samples were loaded on the PacBio Sequel ...Product Name: Reagent Replacement kit for Pippin Prep Gel Cassettes Product Numbers: RBF1510 (Marker R2) RBQ2010 (Marker E1) RSF2010 (Marker X1) RBF7510 (Marker S1) RBF2010 (Marker V1) RUK7510 (Marker U1) RBQ3010 (Marker Q3) RMG7510 (Marker T1) RBE2010 (Marker M1) RHZ7510 (Markter Z1) ...High accuracy is the prerequisite for establishing SV benchmark. For accuracy, we focused on detecting and characterizing large insertions and deletions (Figure 1).By aligning PacBio CLR subreads, we identified 5871 deletions and 6936 insertions (Table 2).The size distribution of deletions displayed 300 bp and 6 kb peaks related to SINE-Alu and LINE elements, respectively (Figure S5), implying ...Scottie's latest target is none other than Michael Jordan. Pippen's beef is centered on the "The Last Dance," the 10-part ESPN produced documentary that captivated the country in 2020 ...Once libraries were completed, they were size selected from 20–50 kb using the Blue Pippin instrument (Sage Science, Beverly MA, USA) to enrich for the longest insert lengths possible. The ...According to the manufacturer' s instructions, an average insert size of 20-kb SMRTbell libraries were constructed using the SMRTbell Template Prep Kit 1.0 (Pacific Biosciences) and Blue Pippin (Labgene Scientific) was then used for fragment size selection.Flexible, movable furniture often feels temporary, missing the comfort factor – not so with Pippin. This single-seat, cone-shaped lounge chair offers a.Safety Data Sheet In accordance with Regulation (EC) No 1272/2008 (REACH) Trade name: BluePippin™ Agarose Gel Cassettes Product No: BDQ3010, BEF2010, BDF2010, BDF1510, PAC20KB, PAC30KB,Bundoran Blue 2018. In Stock. Add To Cart. $60.00 / 750ml. SKU: Bundoran Blue 2018. Quantity: Add To Cart. Petit Verdot 2019. Gold Medal Winner ... Add To Cart. Bin 21 2019. In Stock. Add To Cart. $40.00 / 500ml. SKU: Bin 21 2019. Quantity: Add To Cart « ‹ 1; 2; Of; 2; PIPPIN HILL FARM & VINEYARDS Sign up for our occasional email and be one ...We’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ...Pippin Hill Farm & Vineyards Pollak Vineyards Stinson Vineyards, Charlottesville Monticello Wine Tour Stone Mountain Vineyards, Charlottesville Wine Tours! ... Blue Ridge Mountains, Classic Virginia Wines, Scenic Vistas. East. Features: Trump Winery, Blenheim Vineyards, Keswick Vineyards, Jefferson Vineyards, Mount Ida Tasting Room.Description DNA Gel Cassettes Specifications Downloads Size Select Up To 24 Samples In One Run PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The Pippin platform has since established itself as a key platform for the construction of high-quality libraries for next-gen sequencing. In 2014 Sage Science released a new electrophoresis platform, the SageELF for whole sample fractionation of DNA or proteins, and the PippinHT , a high-throughput version of the BluePippin.Follow the links below to our Youtube page for information or training on Pippin Products: General Sage Science Product Overview, 2020 17:42 SageHLS HMW Library System User Guide. These videos are intended to help users visualize some instructions from the user manual: SageHLS YouTube playlist. Individual videos: HLS-CATCH BRCA1 Demonstration 31:40The PCR cycling conditions were as follows: 12 cycles of 98 °C for 10 s, 54 °C for 15 s, and 68 °C for 1 min. After purification of the second PCR products with AMPure XP magnetic beads, only 300-800 bp fragments with a size suitable for sequencing were selected by the Blue Pippin DNA Size Selection System (Sage Science, Beverly, MA, USA).The Apple Pippin is a defunct open multimedia technology platform, designed by Apple Computer, and marketed as PiPP!N. According to Apple, Pippin was directed at the home market as "an integral part of the consumer audiovisual, stereo, and television environment." ... Sonoran Blue 1996 NA, JP WebViewer with MS Internet Explorer: Non-game, Web ...Unique barcoded primers were ligated to all genomic DNA fragments to enable multiplex sequencing. Genomic DNA fragments between ~365 and 465 bp (415-515 bp after ligating barcoded oligonucleotides) were size-selected with a Blue Pippin DNA fragment size selector (Sage Science, MA, USA).Auntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Run times are as little as 30 minutes. To resolve very large DNA fragments, pulsed-field runs may last several hours. View the Blue Pippin cassette reference chart to explore the DNA size selection options and run times. Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.This instructional video demonstrates the optical calibration procedure for Pippin Prep and BluePippin automated DNA size selection instruments from Sage Sci...Blue Pippin Standard DNA size selection cassette 2% agarose The blue pippin is a machine that is used to size-select DNA fragments from 100bp-50kbp.Target sizes or rangesof sizes are entered in software, and fractions are collected in buffer.He was an eight-time member of the NBA All-Defensive First Team (1992-1999) and the 1994 All-Star MVP. Yet, his career averages of 16.1 points, 6.4 rebounds and 5.2 assists were not staggering ...BN124141 Pippin Product Line Brochure (4 pages): Flyers: BN0205147. Paired-end sequencing flyer. for Illumina platforms. BN0205148. Pippin Pulse flyer. Pulsed-Field for gel boxes. BN0205149.BluePippin Prep. The Pippin Prep is an electrophoresis platform capable of size-selecting DNA fragments and automatically collects the fractions in buffer according to the user-specified size range. It is routinely used to size-select next generation sequencing libraries. It is capable of performing narrow size selections from 50bp to 50kb in ... When it comes to choosing a web host, there are many options available. One of the most popular choices is Blue Hosting. But how does it compare to other web hosts? In this article, we will explore the features and benefits of Blue Hosting ...Then the large fragments of DNA were recovered by Blue Pippin automatic nucleic acid fragment recovery system, and repaired. Barcode was added by PCR-free method of EXP-NBD104 kit (Oxford Nanopore Technologies Company). The size of fragments was detected by AATI automatic capillary electrophoresis instrument, and the samples were isomolar mixed ...Cassette Kit Product Number Cassette Type Software DNA Control Kit Product Number BLG7510 0.75% and Capture 2 – 5 kb Marker D1 CBC7501 BLG7510 0.75% Band Capture 5 – 10 kb Marker D1 CBC7601Sequencing of samples was performed using MiSeq Reagent Kit v3 (600 cycle; 2–300 bp) (Illumina, San Diego, CA, USA). For Long-read WGS, the library preparation and size selection were performed using PacBio Template Preparation 1.0 kit (Pacific Biosciences, Menlo Park, CA, USA) and Blue Pippin (Sage Sciences, Beverly, …Double digest restriction-site associated sequencing (ddRAD-seq) is a flexible and cost-effective strategy for providing in-depth insights into the genetic architecture of germplasm collections.Sage Science, Inc. announced this month, the introduction of the BluePippin, an instrument that automates the size selection and retrieval of DNA for molecular biology research. The BluePippin expands Sage's Pippin line of preparative electrophoresis products by providing the capability to collect larger, kilobase-sized, DNA fragments. The Pippin platform is typically used to construct ...Abstract Root-knot nematodes from the genus Meloidogyne are polyphagous plant endoparasites and agricultural pests of global importance. Here, we report the high-quality genome sequence of Meloidogyne luci population SI-Smartno V13. The resulting genome assembly of M. luci SI-Smartno V13 consists of 327 contigs, with an N50 contig …This instructional video demonstrates the optical calibration procedure for Pippin Prep and BluePippin automated DNA size selection instruments from Sage Sci...The complementary DNA (cDNA) was synthesized using the Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA). Fragments >4 kb were selected using the Blue Pippin Size Selection System protocol (Sage Science, Beverly, MA, USA). The >4 kb cDNA and normal cDNAs were mixed in equimolar amounts for SMRT sequencing.The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites. We therefore investigated the utility of a pre-wash step using a buffered sorbitol solution, prior to DNA extraction using a high salt …Definition of Floppin in the Definitions.net dictionary. Meaning of Floppin. What does Floppin mean? Information and translations of Floppin in the most comprehensive dictionary definitions …Blue Pearmain apple. / 3 see reviews. Description; Reviews (10); Tree register (36) ... ©2023 Orange Pippin Ltd. All rights reserved. You may not reproduce any of ...Page 3 P/N100-377-100-05 1. For each sample and Control Mouse Liver Total RNA, combine the reagents below in separate PCR tubes. For polyA+ RNA, the minimum is 1 ng; total RNA requires 2 ng.lected on a Sage Science Blue Pippin 1.5% cassette (Sage Science, Beverly, USA) to remove DNA molecules <600bp, and amplification-free, paired end libraries constructed usingNews and Events 17-04 Reportage NRC handelsblad: 'Is het een schimmel, is het een virus?Sequencers profileren ziektekiemen razendsnel' hierin vertelt o.a. bio-informaticus Jordy Coolen over het sequencen op de apparaten van ons technology center. 22-12 PacBio long-read sequencing in collaboration with the Senckenberg Research Institute and …4. Follow the manufacturer’s recommendations to set up a run protocol. a. Select the “0.75%DF Marker S1 High-Pass 6–10kb vs3” Cassette Definition File. b. Using the “Range” selection mode, enter a desired “BPstart” value of 10000 and a “BPend” value of 50000. Then, genomic DNA was fragmented with G-tubes and end-repaired to prepare SMRTbell DNA template libraries (with fragment size of >10 Kb selected by the Blue Pippin system). After the average fragment size was estimated on a Bioanalyzer 2100, SMRT sequencing was performed on the Pacific Biosciences Sequel.PippinHT Quick Guide HTC2010 Marker 20B. 1MB. PippinHT Quick Guide HTC1510 Marker 15C. 1MB. PippinHT Quick Guide HPE7510 Marker 75E 6-10Kb and 15-20kb High Pass. 948KB. PippinHT Quick Guide HPF7510 Marker 75F >30-40kb High Pass. 1MB. PippinHT Quick Guide HRT7510 9-30kb Range +T Marker 75E. The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user. 1. Introduction. Human leukocyte antigens (HLA) are highly polymorphic molecules involved in antigen-specific immune responses. Thus, HLA mismatch between donor and recipient can cause alloreactive T-cell activation in the recipients of a transplant.Abstract. The PacBio ® HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10–25 kb and accuracies greater than 99.5%. These accurate long ...Pufferfish are ideal models for vertebrate chromosome evolution studies. The yellowbelly pufferfish, Takifugu flavidus, is an important marine fish species in the aquaculture industry and ecology ...Gels were run using the Pippin Pulse pulsed-˚eld electrophoresis power supply (PPI 0200). H. Collecting Fractions 2. Remove samples using a standard 100-200µl pippette. Aspirate slowly to minimize breakage of large DNA fragments. Samples will be in a Tris-TAPS buffer at a volume of approximately 80µl. 4. Remove the cassette and dispose of ...Libraries were prepared with Illumina TruSeq Stranded mRNA kit starting from 600 ng of total RNA, and samples were fragmented and amplified for 15 PCR cycles. Libraries were size selected with Blue Pippin (Sage Science) using 1.5% gel cassettes and 350-550 bp regions isolated.Dec 10, 2020 · For Nanopore sequencing, large fragments of genomic DNA were obtained using the Blue Pippin automatic nucleic acid fragment recovery system (Sage Science, MA), and barcodes were added using the Nanopore EXP-NBD104 kit. Watch the video below to learn about DNA size selection with Pippin: Whole-Sample Fractionation for DNA or Protein: SageELF. The fully automated SageELF (for Electrophoretic Lateral Fractionator) can be used to select DNA or proteins. It features a cassette system designed to fractionate a whole protein or DNA sample simultaneously into 12 ...blue light for visualization. Do not use ethidium bromide and/or UV light since they can induce DNA damage. The SYBR stains can be easily removed from nucleic acids during the gel-extraction process, e.g., using Qiagen® gel-extraction kits. 2) Purity of your DNA sample: DNA purity can be determined by using the NanoDrop® instrument or other“Blue balls” is slang for that uncomfortable and often painful feeling in a guy’s downstairs region, and it happens to some, not all, guys. But did you know that women can get it too? Blue balls and blue vulvas are very real, and we could a...The Blue Pippin is used to select DNA of certain sizes and effectively remove unwanted fragment sizes. If you intend to use Illumina technology, we will select fragments in a target size range …The end-repaired fragments were size-selected using the Blue Pippin Size-Selection System (Sage Science, MA, USA), and damage-repaired using the SMRTbell Damage Repair Kit (PacBio).As researchers engage larger-scale genomic and transcriptomic studies, the need for high-quality NGS libraries is greater than ever. Now we can provide Pippin size selection for high-throughput workflows so you can easily scale up your sample prep with speedy run times. Features of the PippinHT: 2 x 12-sample gel cassettes can be run at one time.Pearmain. A specimen of 'White Winter Pearmain', collected in Umatilla County, Oregon. A pearmain, also formerly spelled "permain", is a type of apple. The name may once have been applied to a particular variety of apple that kept well, although in more modern times its inclusion in varietal names was, like the term 'Pippin', "largely ...13 thg 8, 2022 ... The Blue Tiger. oil on canvas • 40.64 x 71.12 cm. Horace Pippin - February 22, 1888 - July 6, 1946 Horace Pippin ...Rimex manufactures ColourTex® Blue Pippin metal patterned products on natural and coloured stainless steel. Request a sample today. Specialists in Metal Finishes. ... ColourTex® Bronze Pippin. 1,500mm width and other lengths available upon request. Width: 1.250mm. Length: 2.500mm | 3.000mm. Gauge: 0.6mm MIN | 1.5mm MAX. Sample Request.ID Screen® Bluetongue Competition. Competitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species. BTC-5P / BTC-10P. ID Screen® Bluetongue Milk Indirect. Indirect ELISA for the detection of anti-VP7 antibodies in individual or bulk milk samples. BTSMILK-4P.Description DNA Gel Cassettes Specifications Downloads Size Select Up To 24 Samples In One Run PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. Blue Pippin Release notes: Date: 10/21/21 Version: 6.31 / 6.40 CD 33 What's new from 6.31 / 6.40 CD 33 to 6.31 / 6.40 CD 34 - Added a new cassette definition, "2% DF, Marker V2" for CFD2010 Marker V2 cassettes. Marker V1 is no longer available with new cassette purchases.Welcome to Deep Seq. The sequencing facility offers research scientists access to state of the art high-throughput sequencing technology. Our experienced team of dedicated experimental scientists and bioinformaticians will work with you from the conception of your sequencing experiment to the final analysis of your data.We performed sequence capture on human genomic DNA, using Agilent's SureSelect Human All Exon V6, using a randomly fragmented library which had been size-selected using the Blue Pippin automated gel fractionation system, leaving only fragments above 6 kb. The library was then amplified by PCR prior to performing hybrid capture.Libraries are run through 10-15 cycles of PCR with Kapa Biosystems Library Amplification kits, followed by further size selection with Blue Pippin Prep (Sage Science). Final library quantification is achieved using the Qubit Fluorometer (for concentration) and the Agilent 2100 Bioanalyzer (for library insert size and length).The Blue Pippin (Sage Science Inc., Beverly, MA, USA) was used to select sizes of the fragments (> 15 Kb). After a second round of DNA end repair, the SMRT Bell™ templates were purified for final sequencing with the PacBio Sequel system (PacBio, Menlo Park, CA, USA). Ten SMRT cells were used to obtain a total of 62.6 Gb (~120X) of raw ...BluePippin front panel USB Port Indicator Light: Indicator Light: Blue when instrument is Green when electrophoresis is ready for use (power is on, underway (protocol is running). and …Blue Apron Coupon. Teachers take $160 on their Blue Apron order. Since 1957, GQ has inspired men to look sharper and live smarter with its unparalleled coverage of style, culture, and beyond. From ...Find compatible pollenizers for Blue Pearmain apple trees, based on flowering group, parentage, self-fertility and other attributes. Pre-order for spring 2024 We are now accepting pre-orders for the spring 2024 planting season. ... ©Orange Pippin Ltd 2023 ...Cassette Kit Product Number Cassette Type Software DNA Control Kit Product Number BLG7510 0.75% and Capture 2 – 5 kb Marker D1 CBC7501 BLG7510 0.75% Band Capture 5 – 10 kb Marker D1 CBC7601Pufferfish are ideal models for vertebrate chromosome evolution studies. The yellowbelly pufferfish, Takifugu flavidus, is an important marine fish species in the aquaculture industry and ecology ...
RESOURCE ARTICLE Harnessing the MinION: An example of how to establish long‐read sequencing in a laboratory using challenging plant tissue from Eucalyptus pauciflora Miriam Schalamun1 | Ramawatar Nagar1 | David Kainer1 | Eleanor Beavan1 | David Eccles2 | John P. Rathjen1 | Robert Lanfear1 | Benjamin Schwessinger1 1Research School of Biology, The Australian National University, Acton, ACT .... Ms in education meaning
BLUE-PIPPIN INSTALLATION PROCEDURE 3.1 Packaging and Components The system is shipped in two boxes: one will contain the BluePippin instrument and accessories, and the second box will contain the computer monitor in the manufacturer's original packaging. With the boxes in the upright position, open and confirm that the following items are ...Blippi visits the pumpkin playground for kids! Blippi special Halloween videos for Kids. In this educational video for kids Blippi teaches us about Halloween...The BluePippin is a preparative electrophoresis platform for collection of size-selected DNA or protein samples. The system automates DNA or protein size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer.Watch the video below to learn about DNA size selection with Pippin: Whole-Sample Fractionation for DNA or Protein: SageELF. The fully automated SageELF (for Electrophoretic Lateral Fractionator) can be used to select DNA or proteins. It features a cassette system designed to fractionate a whole protein or DNA sample simultaneously into 12 ...The Blue Pippin has both standard and pulse field electrophoresis capability permitting a large range of fragment size selection. Cassettes can run up to 5 samples. Unused sample lanes can be used at a later time in some cases. Gel cassettes available are: 3% agarose 100-250bp isolation 2.5% agarose 100-600bp 1.5% agarose 250bp – 1.5KbAutomated, preparative gel electrophoresis systems, used for accurate and reproducible size selection/collection of DNA (or cDNA) fragments, for the preparation of libraries for next generation sequencing (NGS) The supplier does not provide quotations for this product through SelectScience. Browse these related products instead.Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Background. Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes.No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop pests.In contrast, genomic information for crop pests is far ...The selected size for this method was 314-414 bp using the Blue Pippin system. We predicted that there were 117,528 SLAF tags distributed in the genome with 6.19% located in repetitive regions. To further evaluate the efficiency of the restriction enzyme digestion scheme, Arabidopsis thaliana was selected as an example.The ColourTex® stainless steel range is unique whereby the colours and tones of the product varies in different lighting conditions. This is known as a 'living' colour when viewed from different angles, also known as metamerism. The ColourTex® process can be combined with the surface finishes of mirror, satin and other polishes, Granex ...Description DNA Gel Cassettes Specifications Downloads Size Select Up To 24 Samples In One Run PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. We size selected using a Sage Science Blue Pippin for 376-476 bp fragments (i.e., 300-400 bp fragments, plus 76 bp of P1 and P2 adapters), performed final PCRs using 12 cycles, and assessed final ....